Protein Parameter Calculator

This script calculates molar absorptivities (extinction coefficients) at 205 nm and 280 nm from an amino acid sequence. It also calculates the molecular weight for various universal isotopic labeling schemes.

Enter your amino acid sequence (in single letters) in the box below. It can be upper- or lower-case and in any format (the script will ignore any spaces, numbers, symbols, etc.), but any other text will be interpreted as amino acids. Input is limited to 5,000 characters in total.

Reference (for further information and for citation):

Anthis N.J. & Clore G. M. (2013) Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm, Protein Science 22, 851-8. pubmed pdf